Our laboratory aims to elucidate the molecular mechanism underlying DNA double-strand break (DSB) repair, which is critical for maintaining genome integrity. Defects in DSB repair lead to severe mutations, including deletions and chromosomal translocations, which cause cell death. Introducing such severe mutations is important for killing cancer cells via radio/chemotherapy. In addition, DSB repair and signaling is involved in many biological events such as tumorigenesis, immunity, protection against virus, and biological development.
We are currently investigating the interplay between non-homologous end joining (NHEJ) and homologous recombination (HR), which are major DSB repair pathways. We have recently attempted to visualize a repair event at a single DSB using super-resolution microscope 3D structured-illumination microscopy (SIM). We have also conducted several ongoing projects with radiation oncologists on combined radio- and immunotherapy.